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Foundations of the Cellular Secretome

Why the Cellular Secretome Matters

What Real-Time Secretome Biosensing Is — and Is Not

Bulk, Endpoint, and Single-Cell Measurements

Optical Biosensing Platforms and Techniques

Microengraving and Antibody Arrays

Fluorescence Imaging of Secretion

Label-Free Plasmonic Biosensing

Assay Dynamics, Confounders, and Complex Targets

Transport, Capture, and Calibration

Multiplexing and Polyfunctionality

Extracellular Vesicles and Other Secretions

Translation, Evidence, and Decision-Making

Evidence: What Counts as Strong Enough?

Translation Barriers That Often Get Underestimated

What Stakeholders Should Ask

Applying Decision Frameworks via Case Scenarios

Quizzes

Quiz: Foundations of the Cellular Secretome

Quiz: Understanding Measurement Strategies

Quiz: Microengraving and Antibody Platforms

Quiz: Fluorescence-Based Optical Biosensing

Quiz: Label-Free Plasmonic Techniques

Quiz: Transport, Kinetics, and Capture Chemistry

Quiz: Multiplexing and Cellular Polyfunctionality

Quiz: Measuring Extracellular Vesicles

Quiz: Evaluating Evidence and Reproducibility

Quiz: Translation Barriers and Stakeholder Perspectives

Surveys and Feedback

Survey: Course Expectations and Professional Background

Survey: Clarity of Foundational Concepts

Survey: Relevance of Optical Platform Instruction

Survey: Understanding of Assay Confounders

Survey: Evaluation of Multiplexing Content

Survey: Feedback on Extracellular Vesicle Measurement

Survey: Applicability to Research and Bioprocessing

Survey: Case Scenarios Practicality

Survey: Stakeholder Framework Utility

Survey: Final Comprehensive Course Evaluation

Unlock the dynamic world of cellular communication with our comprehensive course, 'Real-Time Single-Cell Secretome Analysis Using Optical Biosensors. ' The cellular secretome—comprising cytokines, antibodies, growth factors, and extracellular vesicles—is the critical language cells use to interact, driving systemic physiology, disease progression, and therapeutic responses.

Unlock the dynamic world of cellular communication with our comprehensive course, 'Real-Time Single-Cell Secretome Analysis Using Optical Biosensors.' The cellular secretome—comprising cytokines, antibodies, growth factors, and extracellular vesicles—is the critical language cells use to interact, driving systemic physiology, disease progression, and therapeutic responses. Traditional bulk and endpoint assays often obscure vital cell-to-cell heterogeneity and rare hyper-secretor phenotypes. This course bridges that gap by diving deep into cutting-edge real-time, single-cell optical biosensing technologies.

Throughout this advanced program, you will explore the foundational principles of the secretome and discover why single-cell resolution is revolutionizing immunology, cancer research, and regenerative medicine. We will guide you through state-of-the-art optical platforms, including microengraving, Total Internal Reflection Fluorescence (TIRF) imaging, and label-free plasmonic biosensing (SPR/LSPR). Beyond the technology, you will learn to navigate complex assay dynamics, such as transport confounders, capture kinetics, and multiplexing challenges.

Designed for researchers, bioprocess engineers, and data scientists, this course doesn't just teach theory—it equips you with practical decision-making frameworks. You will learn to evaluate evidence, overcome severe translational barriers, and answer the critical questions stakeholders ask when moving biosensors from prototype to standard lab use or cell-therapy production. Join us to master the techniques that are shaping the future of targeted cell therapies and biomanufacturing.

57. Real-Time Single-Cell Secretome Analysis Using Optical Biosensors

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            <p>The cellular secretome, comprising a diverse array of cytokines, antibodies, growth factors, and extracellular vesicles, serves as the fundamental conduit for intercellular communication and systemic physiological regulation. Historically, the characterization of these critical secretory molecules has relied heavily on bulk supernatant assays and static endpoint measurements. While foundational, these traditional methodologies inherently obscure cell-to-cell heterogeneity and mask the presence of rare, hyper-secreting phenotypes that are often pivotal in immunology, oncology, and regenerative medicine. To fully harness the potential of targeted cell therapies and advanced biomanufacturing, a paradigm shift toward high-resolution, dynamic monitoring is essential. Real-time single-cell secretome analysis addresses this critical analytical gap by enabling the continuous, time-resolved tracking of secretory events at the individual cell level, thereby revealing the true kinetic trajectories, burst sizes, and polyfunctional states of complex cellular populations.</p>

<p>This comprehensive curriculum systematically explores the intersection of microfluidics, optical engineering, and cellular biology to equip interdisciplinary professionals with the expertise necessary to implement and critically evaluate advanced biosensing technologies. Participants will examine state-of-the-art optical platforms, including antibody-barcode microengraving, surface-confined fluorescence modalities like Total Internal Reflection Fluorescence (TIRF), and label-free plasmonic biosensing techniques such as Surface Plasmon Resonance (SPR). Beyond instrumentation, the coursework rigorously addresses the complex biophysical dynamics that govern assay performance, analyzing how analyte diffusion, capture kinetics, and sensor cross-reactivity impact data interpretation. By evaluating severe translation barriers, reproducibility standards, and structured stakeholder decision frameworks, researchers, bioprocess engineers, and analytical scientists will be prepared to navigate the multifaceted challenges of transitioning these innovative optical biosensors from prototype development to robust, commercial-scale application.</p>
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