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Foundations of Mechanobiology and Biophotonics

1. Why Mechanobiology Matters

3. What Mechanobiology Biophotonics Is — and Is Not

Cell-Matrix Interactions and Cytoskeletal Dynamics

4. Cell–Matrix Adhesion and Mechanotransduction

Actomyosin Contractility and Cell Migration

12. Matrix Properties, Dimensionality, and Cell Fate

Measuring Forces and Adhesion Nanostructure

6. Traction-Force Microscopy

11. Optical Tweezers

7. FRET-Based Molecular Tension Sensors

8. Super-Resolution Imaging of Adhesions

Optical Assessment of Matrix Organization and Intracellular Signaling

9. SHG Imaging of Matrix Organization

10. Brillouin Microscopy and Optical Elastography

Live-Cell Reporters and Mechanotransduction Readouts

Translation, Validation, and Decision Frameworks

13. Evidence: What Counts as Strong Enough?

14. Translation Barriers That Often Get Underestimated

15. What Stakeholders Should Ask

16. Case Scenarios

Quizzes

Quiz 1: Fundamentals of Mechanobiology

Quiz 2: Adhesion and Cytoskeleton Dynamics

Quiz 3: Matrix Mechanics and Dimensionality

Quiz 4: Traction-Force Microscopy

Quiz 5: Optical Tweezers and Single-Molecule Forces

Quiz 6: Molecular Tension Sensors

Quiz 7: Advanced Imaging of Adhesions

Quiz 8: Optical Elastography and Matrix Organization

Quiz 9: Overcoming Translational Barriers

Quiz 10: Stakeholder Questions and Data Interpretation

Surveys and Feedback

Survey 1: Learner Background and Demographics

Survey 2: Relevance of Traction-Force Microscopy

Survey 3: Perspectives on FRET Sensors

Survey 4: Advanced Imaging Accessibility

Survey 5: Biophotonics Translation Barriers

Survey 6: Evaluating Elastography Methods

Survey 7: Interdisciplinary Communication

Survey 8: Course Pacing and Complexity

Survey 9: Practical Application of Decision Frameworks

Survey 10: Overall Course Satisfaction and Future Needs

Welcome to "Biophotonics for Mechanobiology: Measuring Cell Forces and Matrix Mechanics," a comprehensive online course designed to bridge the gap between physical sciences and biology. Mechanobiology explores how physical forces and changes in cell or tissue mechanics contribute to development, physiology, and disease.

Welcome to "Biophotonics for Mechanobiology: Measuring Cell Forces and Matrix Mechanics," a comprehensive online course designed to bridge the gap between physical sciences and biology. Mechanobiology explores how physical forces and changes in cell or tissue mechanics contribute to development, physiology, and disease. In this cutting-edge course, you will dive deep into the fundamental pathways by which physical cues are translated into biochemical signals, and discover how matrix mechanics impact disease progression, such as in cancer and fibrosis.

Through a detailed exploration of biophotonics, you will learn how to accurately measure cell-generated forces and matrix mechanics using advanced optical approaches. We will demystify complex techniques like Traction-Force Microscopy (TFM), Optical Tweezers, FRET-based molecular tension sensors, and Super-Resolution Imaging. Furthermore, you will explore the optical assessment of matrix organization using Second Harmonic Generation (SHG) and Brillouin microscopy.

Beyond the technical methodologies, this course emphasizes real-world translation and validation. You will learn how to navigate translation barriers, evaluate evidence, and apply rigorous decision frameworks to ensure your optical data supports causal mechanistic claims. Whether you are a biologist, a data scientist, or an imaging specialist, this course will equip you with the interdisciplinary knowledge needed to critically analyze cellular forces, matrix interactions, and mechanotransduction readouts. Join us to master the optical tools that are revolutionizing our understanding of cellular mechanics!

60. Biophotonics for Mechanobiology: Measuring Cell Forces and Matrix Mechanics

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            <p>The dynamic interplay between cellular forces and extracellular matrix mechanics is a fundamental driver of biological processes, influencing everything from stem cell differentiation to the progression of complex diseases such as cancer and fibrosis. Understanding these mechanobiological phenomena requires precise, non-destructive methodologies capable of probing the physical microenvironment at the nanoscale. This course provides a comprehensive exploration of biophotonics as a critical toolset for measuring cell-generated forces and matrix mechanics. By examining the foundational principles of mechanosensing and mechanotransduction, participants will gain a rigorous understanding of how physical cues are translated into biochemical signals through cell-matrix adhesions, actomyosin contractility, and cytoskeletal dynamics.</p>

<p>To bridge the gap between biological observation and quantitative biophysics, the curriculum systematically evaluates advanced optical techniques, including traction-force microscopy, optical tweezers, FRET-based molecular tension sensors, and super-resolution imaging. Furthermore, the program delves into the optical assessment of matrix organization and intracellular signaling utilizing Second Harmonic Generation (SHG) and Brillouin microscopy. Crucially, the course emphasizes the translation and validation of these optical proxies, equipping interdisciplinary teams—ranging from cell biologists and biophysicists to data scientists and imaging-core staff—with robust decision frameworks. Participants will learn to critically analyze inverse-problem assumptions, mitigate translational barriers, and establish the rigorous evidence required to substantiate mechanistic claims in modern mechanobiology research.</p>
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